Liu, ZX.1, Zhang, S.1, Zhu, HZ.1, Chen, ZH.1, Yang, Y.1, Li, LQ.1, Lei, Y., Liu, Y., Li, DY., Sun, A., Li, CP., Tan, SQ., Wang, GL., Shen, JY., Jin, S., Gao, C., Liu, JJ.* (2024). Hydrolytic endonucleolytic ribozyme (HYER) is programmable for sequence-specific DNA cleavage. Science, 383, 495.
Zou, J., Huang, Y., Gao, C., Wang, K. (2023). Unlocking crop diversity: enhancing variations through genome editing. Science Bulletin doi: https://doi.org/10.1016/j.scib.2023.12.013.
Hu, J., Sun, Y., Li, B., Liu, Z., Wang, Z., Gao, Q., Guo, M., Liu, G., Zhao, K., Gao, C. (2023). Strand-preferred base editing of organellar and nuclear genomes using CyDENT. Nature Biotechnology doi: 10.1038/s41587-023-01910-9.
Xing, S., Sun, Y., Li, B., Li, H., Zhao, KT., Chen, K.*, Gao, C.* (2023). Anthocyanin-assisted Agrobacterium infiltration for the rapid evaluation of genome editing efficiencies across multiple plant species. National Science Open 2, 20220052.
Huang, J., Lin, Q., Fei, H., He, Z., Xu, H., Li, Y., Qu, K., Han, P., Gao, Q., Li, B. et al. (2023). Discovery of deaminase functions by structure-based protein clustering. Cell 186, 3182-3195.
⠂One recommendation in F1000 Prime
Sun, C., Lei, Y., Li, B., Gao, Q., Li, Y., Cao, W., Yang, C., Li, H., Wang, Z., Li, Y. et al. (2023). Precise integration of large DNA sequences in plant genomes using PrimeRoot editors. Nature Biotechnoloy 42, 316-327.
⠂Two recommendations in F1000 Prime
⠂Commentary in Trends in Genetics by M. A. Mahmood and J. R. Greenwood
⠂Commentary in Trends in Plant Science by C. Pan and Y. Qi
Xue, C., Gao, C. (2023). Engineered upstream open reading frames predictably downregulate mRNA translation in plants. Nature Biotechnology 41, 1694–1695.
Xue, C., Qiu, F., Wang, Y., Li, B., Zhao, K.T., Chen, K., and Gao, C. (2023). Tuning plant phenotypes by precise, graded downregulation of gene expression. Nature Biotechnology 41, 1758–1764.
⠂Commentary in Science China Life Sciences by G. Luo and M. Palmgren
Zhang, X.E., Liu, C., Dai, J., Yuan, Y., Gao, C., Feng, Y., Wu, B., Wei, P., You, C., Wang, X., et al. (2023). Enabling technology and core theory of synthetic biology. Science China Life Sciences 66, 1742-1785.
Gao, C., Kikulwe, E., Kuzma, J., Lema, M., Lidder, P., Robinson, J., Wessler, J., and Zhao, K. (2022). Gene editing and agrifood systems. United Nations Food and Agriculture Organization (FAO) doi: 10.4060/cc3579en.
Xu, F., Tang, J., Wang, S., Cheng, X., Wang, H., Ou, S., Gao, S., Li, B., Qian, Y., Gao, C., et al. (2022). Antagonistic control of seed dormancy in rice by two bHLH transcription factors. Nature Genetics 54, 1972-1982.
Wei, W.S., and Gao, C.X. (2022). Gene editing: from technologies to applications in research and beyond. Science China-Life Sciences 65, 657-659.
Wang, N., Fan, X., He, M., Hu, Z., Tang, C., Zhang, S., Lin, D., Gan, P., Wang, J., Huang, X., et al. (2022). Transcriptional repression of TaNOX10 by TaWRKY19 compromises ROS generation and enhances wheat susceptibility to stripe rust. Plant Cell 34, 1784-1803.
Luo, G., Najafi, J., Correia, P.M.P., Trinh, M.D.L., Chapman, E.A., Østerberg, J.T., Thomsen, H.C., Pedas, P.R., Larson, S., Gao, C., et al. (2022). Accelerated Domestication of New Crops: Yield is Key. Plant Cell Physiology 63, 1624-1640.
Zong, Y., Liu, Y., Xue, C., Li, B., Li, X., Wang, Y., Li, J., Liu, G., Huang, X., Cao, X., et al. (2022). An engineered prime editor with enhanced editing efficiency in plants. Nature Biotechnology 40,1394-1402.
⠂Commentary in Trends in Plant Science by S. Mansoor et al.
Zong, Y., Liu, Y., Xue, C., Li, B., Li, X., Wang, Y., Li, J., Liu, G., Huang, X., Cao, X., et al. (2022). Author Correction: An engineered prime editor with enhanced editing efficiency in plants. Nature Biotechnology 40, 1412.
Zhang, R., Chen, S., Meng, X., Chai, Z., Wang, D., Yuan, Y., Chen, K., Jiang, L., Li, J., and Gao, C. (2022). Erratum to: Generating broad-spectrum tolerance to ALS-inhibiting herbicides in rice by base editing. Science China Life sciences 65, 1270.
Li, S., Lin, D., Zhang, Y., Deng, M., Chen, Y., Lv, B., Li, B., Lei, Y., Wang, Y., Zhao, L., et al. (2022). Genome-edited powdery mildew resistance in wheat without growth penalties. Nature 602, 455-460.
⠂Preview by J. K. M. Brown and B. B. H. Wulff in Cell
⠂News by E. Stokstad in Science
⠂News by S. Mallapaty in Nature
⠂News & Views by P. D. Spanu in Nature Plants
⠂Research Highlights by X. Deng and X. Cao in Nature Reviews Genetics
⠂Spotlight in Trends in Plant Science by J. Najafi and M. Palmgren
⠂Commentary by J. R. Greenwood and J. P. Rathjen in Current Biology
⠂Three recommendations in F1000 Prime
Liu, G., Lin, Q., Jin, S., and Gao, C. (2022). The CRISPR-Cas toolbox and gene editing technologies. Molecular cell 82, 333-347.
Luo, G., Li, B., and Gao, C. (2022). Protoplast Isolation and Transfection in Wheat. Methods in molecular biology 2464, 131-141.
Luo, J., Rouse, M.N., Hua, L., Li, H., Li, B., Li, T., Zhang, W., Gao, C., Wang, Y., Dubcovsky, J., et al. (2022). Identification and characterization of Sr22b, a new allele of the wheat stem rust resistance gene Sr22 effective against the Ug99 race group. Plant biotechnology journal 20, 554-563.
Zhang, R., Chen, S., Meng, X., Chai, Z., Wang, D., Yuan, Y., Chen, K., Jiang, L., Li, J., and Gao, C. (2021). Generating broad-spectrum tolerance to ALS-inhibiting herbicides in rice by base editing. Science China Life sciences 64, 1624-1633.
Yu, H., Lin, T., Meng, X., Du, H., Zhang, J., Liu, G., Chen, M., Jing, Y., Kou, L., Li, X., et al. (2021). A route to de novo domestication of wild allotetraploid rice. Cell 184, 1156-1170.
⠂ Two recommendations in F1000 Prime
⠂ Preview by X.-G. Zhu and J.-K. Zhu in Cell
⠂ News & Views by D. R. Wang in Nature
⠂ Research Highlights by X. Xie and Y.-G. Liu in National Science Review
Xu, Y., Lin, Q., Li, X., Wang, F., Chen, Z., Wang, J., Li, W., Fan, F., Tao, Y., Jiang, Y., et al. (2021). Fine-tuning the amylose content of rice by precise base editing of the Wx gene. Plant biotechnology journal 19, 11-13.
Xu, S., Dong, Q., Deng, M., Lin, D., Xiao, J., Cheng, P., Xing, L., Niu, Y., Gao, C., Zhang, W., et al. (2021). The vernalization-induced long non-coding RNA VAS functions with the transcription factor TaRF2b to promote TaVRN1 expression for flowering in hexaploid wheat. Molecular plant 14, 1525-1538.
Qiu, F., Xing, S., Xue, C., Liu, J., Chen, K., Chai, T., and Gao, C. (2021). Transient expression of a TaGRF4-TaGIF1 complex stimulates wheat regeneration and improves genome editing. Science China Life sciences 65, 731-738.
Luo, G., Shen, L., Zhao, S., Li, R., Song, Y., Song, S., Yu, K., Yang, W., Li, X., Sun, J., et al. (2021). Genome-wide identification of seed storage protein gene regulators in wheat through coexpression analysis. The Plant journal 108, 1704-1720.
Luo, G., Shen, L., Song, Y., Yu, K., Ji, J., Zhang, C., Yang, W., Li, X., Sun, J., Zhan, K., et al. (2021). The MYB family transcription factor TuODORANT1 from Triticum urartu and the homolog TaODORANT1 from Triticum aestivum inhibit seed storage protein synthesis in wheat. Plant biotechnology journal 19, 1863-1877.
Lin, Q., Zhu, Z., Liu, G., Sun, C., Lin, D., Xue, C., Li, S., Zhang, D., Gao, C., Wang, Y., et al. (2021). Genome editing in plants with MAD7 nuclease. Journal of genetics and genomics 48, 444-451.
Lin, Q., Jin, S., Zong, Y., Yu, H., Zhu, Z., Liu, G., Kou, L., Wang, Y., Qiu, J.L., Li, J., et al. (2021). High-efficiency prime editing with optimized, paired pegRNAs in plants. Nature biotechnology 39, 923-927.
⠂Research Highlights by L. Tang in Nature Methods
Li, T., Hu, J., Sun, Y., Li, B., Zhang, D., Li, W., Liu, J., Li, D., Gao, C., Zhang, Y., et al. (2021). Highly efficient heritable genome editing in wheat using an RNA virus and bypassing tissue culture. Molecular plant 14, 1787-1798.
Jin, S., Lin, Q., Luo, Y., Zhu, Z., Liu, G., Li, Y., Chen, K., Qiu, J.L., and Gao, C. (2021). Genome-wide specificity of prime editors in plants. Nature biotechnology 39, 1292-1299.
⠂Research Highlights by L. Tang inNature Methods
Jin, S., Gao, Q., and Gao, C. (2021). An unbiased method for evaluating the genome-wide specificity of base editors in rice. Nature protocols 16, 431-457.
Li, C., Zhang, R., Meng, X., Chen, S., Zong, Y., Lu, C., Qiu, J.L., Chen, Y.H., Li, J., and Gao, C. (2020). Targeted, random mutagenesis of plant genes with dual cytosine and adenine base editors. Nature biotechnology 38, 875-882.
⠂Three recommendations in F1000 Prime
⠂Commentary in Science China Life Sciences by N. Capdeville, P. Schindele and H. Puchta
⠂Commentary in Hereditas by F. Hu and K. Wang
Lin, Q., Zong, Y., Xue, C., Wang, S., Jin, S., Zhu, Z., Wang, Y., Anzalone, A.V., Raguram, A., Doman, J.L., et al. (2020). Prime genome editing in rice and wheat. Nature biotechnology 38, 582-585.
⠂One recommendation in F1000 Prime
⠂Spotlight by M. Marzec and G. Hensel in Trends in Plant Science
Si, X., Zhang, H., Wang, Y., Chen, K., and Gao, C. (2020). Manipulating gene translation in plants by CRISPR-Cas9-mediated genome editing of upstream open reading frames. Nature protocols 15, 338-363.
DeHaan, L., Larson, S., López-Marqués, R.L., Wenkel, S., Gao, C., and Palmgren, M. (2020). Roadmap for Accelerated Domestication of an Emerging Perennial Grain Crop. Trends in plant science 25, 525-537.
López-Marqués, R.L., Nørrevang, A.F., Ache, P., Moog, M., Visintainer, D., Wendt, T., Østerberg, J.T., Dockter, C., Jørgensen, M.E., Salvador, A.T., et al. (2020). Prospects for the accelerated improvement of the resilient crop quinoa. Journal of experimental botany 71, 5333-5347.
Li, C., Zong, Y., Jin, S., Zhu, H., Lin, D., Li, S., Qiu, J.L., Wang, Y., and Gao, C. (2020). SWISS: multiplexed orthogonal genome editing in plants with a Cas9 nickase and engineered CRISPR RNA scaffolds. Genome biology 21, 141.
Wang, H., Sun, S., Ge, W., Zhao, L., Hou, B., Wang, K., Lyu, Z., Chen, L., Xu, S., Guo, J., et al. (2020). Horizontal gene transfer of Fhb7 from fungus underlies Fusarium head blight resistance in wheat. Science 368, 844-851.
Zhang, Y., Pribil, M., Palmgren, M., and Gao, C. (2020). A CRISPR way for accelerating improvement of food crops. Nature Food 1, 200-205.
Chen, K., Gao, C. (2020). Genome-edited crops: how to move them from laboratory to market. Frontiers of Agricultural Science and Engineering 7, 181–187.
Zhang, Y., Ran, Y., Nagy, I., Lenk, I., Qiu, J.L., Asp, T., Jensen, C.S., and Gao, C. (2020). Targeted mutagenesis in ryegrass (Lolium spp.) using the CRISPR/Cas9 system. Plant biotechnology journal 18, 1854-1856.
Fan, R., Chai, Z., Xing, S., Chen, K., Qiu, F., Chai, T., Qiu, J.L., Zhang, Z., Zhang, H., and Gao, C. (2020). Shortening the sgRNA-DNA interface enables SpCas9 and eSpCas9(1.1) to nick the target DNA strand. Science China Life sciences 63, 1619-1630.
Zhu, H., Li, C., and Gao, C. (2020). Applications of CRISPR-Cas in agriculture and plant biotechnology. Nature reviews Molecular cell biology 21, 661-677.
Xing, S., Chen, K., Zhu, H., Zhang, R., Zhang, H., Li, B., and Gao, C. (2020). Fine-tuning sugar content in strawberry. Genome biology 21, 230.
Jin, S., Fei, H., Zhu, Z., Luo, Y., Liu, J., Gao, S., Zhang, F., Chen, Y.H., Wang, Y., and Gao, C. (2020). Rationally Designed APOBEC3B Cytosine Base Editors with Improved Specificity. Molecular cell 79, 728-740.
⠂Commentary in Molecular Cell by S. Collins and C. L. Beisel
Xie, H., Ge, X., Yang, F., Wang, B., Li, S., Duan, J., Lv, X., Cheng, C., Song, Z., Liu, C., et al. (2020). High-fidelity SaCas9 identified by directional screening in human cells. PLoS biology 18, e3000747.
Wang, S., Zong, Y., Lin, Q., Zhang, H., Chai, Z., Zhang, D., Chen, K., Qiu, J.L., and Gao, C. (2020). Precise, predictable multi-nucleotide deletions in rice and wheat using APOBEC-Cas9. Nature biotechnology 38, 1460-1465.
Cao, X., Dong, Z., Tian, D., Dong, L., Qian, W., Liu, J., Liu, X., Qin, H., Zhai, W., Gao, C., et al. (2020). Development and characterization of marker-free and transgene insertion site-defined transgenic wheat with improved grain storability and fatty acid content. Plant biotechnology journal 18, 129-140.
Ji, X., Wang, D., and Gao, C. (2019). CRISPR editing-mediated antiviral immunity: a versatile source of resistance to combat plant virus infections. Science China Life sciences 62, 1246-1249.
Gao, C. (2019). Precision plant breeding using genome editing technologies. Transgenic research 28, 53-55.
Hickey, L.T., A, N.H., Robinson, H., Jackson, S.A., Leal-Bertioli, S.C.M., Tester, M., Gao, C., Godwin, I.D., Hayes, B.J., and Wulff, B.B.H. (2019). Breeding crops to feed 10 billion. Nature biotechnology 37, 744-754.
Liu, G., Yin, K., Zhang, Q., Gao, C., and Qiu, J.L. (2019). Modulating chromatin accessibility by transactivation and targeting proximal dsgRNAs enhances Cas9 editing efficiency in vivo. Genome biology 20, 145.
He, X., Wang, Y., Yang, F., Wang, B., Xie, H., Gu, L., Zhao, T., Liu, X., Zhang, D., Ren, Q., et al. (2019). Boosting activity of high-fidelity CRISPR/Cas9 variants using a tRNA(Gln)-processing system in human cells. The Journal of biological chemistry 294, 9308-9315.
Zhang, R., Liu, J., Chai, Z., Chen, S., Bai, Y., Zong, Y., Chen, K., Li, J., Jiang, L., and Gao, C. (2019). Generation of herbicide tolerance traits and a new selectable marker in wheat using base editing. Nature plants 5, 480-485.
Li, J., and Gao, C. (2019). Preface to the special topic on genome editing research in China. National science review 6, 389-390.
Jin, S., Zong, Y., Gao, Q., Zhu, Z., Wang, Y., Qin, P., Liang, C., Wang, D., Qiu, J.L., Zhang, F., et al. (2019). Cytosine, but not adenine, base editors induce genome-wide off-target mutations in rice. Science 364, 292-295.
⠂ Research Highlights by K. H. Wrighton inNature Reviews Genetics
⠂ Research Highlights by G. Zhang, Z. Zhou and W. Wei inCell Research
⠂ News & Views by J. Tang, L. Chen and Y.-G. Liu inNature Plants
⠂ Research Highlights by H. Xin, T. Wan and Y. Ping inSignal Transduction and Targeted Therapy
⠂ Comment by M. R. Willmann inCRISPR Journal
⠂ Perspective by H. R. Kempton and L. S. Qi inScience
⠂ News by J. Cohen in Science
⠂ News & Views by J Chen, B. Yang and L. Yang in Nature Biotechnology
Wang, H., Li, J., Li, W., Gao, C., and Wei, W. (2018). CRISPR twins: a condemnation from Chinese academic societies. Nature 564, 345.
Zhang, Y., Li, D., Zhang, D., Zhao, X., Cao, X., Dong, L., Liu, J., Chen, K., Zhang, H., Gao, C., et al. (2018). Analysis of the functions of TaGW2 homoeologs in wheat grain weight and protein content traits. The Plant journal 94, 857-866.
Liang, Z., Chen, K., Zhang, Y., Liu, J., Yin, K., Qiu, J.L., and Gao, C. (2018). Genome editing of bread wheat using biolistic delivery of CRISPR/Cas9 in vitro transcripts or ribonucleoproteins. Nature protocols 13, 413-430.
Gao, C. (2018). The future of CRISPR technologies in agriculture. Nature reviews Molecular cell biology 19, 275-276.
Zong, Y., Song, Q., Li, C., Jin, S., Zhang, D., Wang, Y., Qiu, J.L., and Gao, C. (2018). Efficient C-to-T base editing in plants using a fusion of nCas9 and human APOBEC3A. Nature biotechnology 36, 950-953.
⠂Commentary in Science China Life Sciences by J.-F. Li
Xue, C., Zhang, H., Lin, Q., Fan, R., and Gao, C. (2018). Manipulating mRNA splicing by base editing in plants. Science China Life sciences 61, 1293-1300.
⠂Cover story
⠂Best Paper in Science China Life Sciences
Zhang, D., Xu, Z., Cao, S., Chen, K., Li, S., Liu, X., Gao, C., Zhang, B., and Zhou, Y. (2018). An Uncanonical CCCH-Tandem Zinc-Finger Protein Represses Secondary Wall Synthesis and Controls Mechanical Strength in Rice. Molecular plant 11, 163-174.
Wang, M., Wang, S., Liang, Z., Shi, W., Gao, C., and Xia, G. (2018). From Genetic Stock to Genome Editing: Gene Exploitation in Wheat. Trends in biotechnology 36, 160-172.
Liang, Z., Chen, K., Yan, Y., Zhang, Y., and Gao, C. (2018). Genotyping genome-edited mutations in plants using CRISPR ribonucleoprotein complexes. Plant biotechnology journal 16, 2053-2062.
Meng, X., Hu, X., Liu, Q., Song, X., Gao, C., Li, J., and Wang, K. (2018). Robust genome editing of CRISPR-Cas9 at NAG PAMs in rice. Science China Life sciences 61, 122-125.
Bhowmik, P., Ellison, E., Polley, B., Bollina, V., Kulkarni, M., Ghanbarnia, K., Song, H., Gao, C., Voytas, D.F., and Kagale, S. (2018). Targeted mutagenesis in wheat microspores using CRISPR/Cas9. Scientific reports 8, 6502.
Zhang, H., Si, X., Ji, X., Fan, R., Liu, J., Chen, K., Wang, D., and Gao, C. (2018). Genome editing of upstream open reading frames enables translational control in plants. Nature biotechnology 36, 894-898.
⠂Commentary in National Science Review by F. Zhang and D. Voytas
Guo, L., Cao, X., Liu, Y., Li, J., Li, Y., Li, D., Zhang, K., Gao, C., Dong, A., and Liu, X. (2018). A chromatin loop represses WUSCHEL expression in Arabidopsis. The Plant journal 94, 1083-1097.
Li, C., Zong, Y., Wang, Y., Jin, S., Zhang, D., Song, Q., Zhang, R., and Gao, C. (2018). Expanded base editing in rice and wheat using a Cas9-adenosine deaminase fusion. Genome biology 19, 59.
Li, T., Yang, X., Yu, Y., Si, X., Zhai, X., Zhang, H., Dong, W., Gao, C., and Xu, C. (2018). Domestication of wild tomato is accelerated by genome editing. Nature biotechnology 36, 1160-1163.
⠂ One recommendation in F1000 Prime
⠂ Spotlight by Muhammad Zuhaib Khan, Syed Shan-e-Ali Zaidi, Imran Amin & Shahid Mansoor in Trends in Plant Science, 2019, 24: 293-296
⠂ Editorials in Nature, 2018, 562: 8
Wang, Y., Zong, Y., and Gao, C. (2017). Targeted Mutagenesis in Hexaploid Bread Wheat Using the TALEN and CRISPR/Cas Systems. Methods in molecular biology 1679, 169-185.
Jiao, R., and Gao, C. (2017). Anything impossible with CRISPR/Cas9? Science China Life sciences 60, 445-446.
Zhang, Y., and Gao, C. (2017). Recent advances in DNA-free editing and precise base editing in plants. Emerging topics in life sciences 1, 161-168.
Wang, Y., Yu, H., Tian, C., Sajjad, M., Gao, C., Tong, Y., Wang, X., and Jiao, Y. (2017). Transcriptome Association Identifies Regulators of Wheat Spike Architecture. Plant physiology 175, 746-757.
Tu, M., Lin, L., Cheng, Y., He, X., Sun, H., Xie, H., Fu, J., Liu, C., Li, J., Chen, D., et al. (2017). A 'new lease of life': FnCpf1 possesses DNA cleavage activity for genome editing in human cells. Nucleic acids research 45, 11295-11304.
Zhang, D., Zhang, H., Li, T., Chen, K., Qiu, J.L., and Gao, C. (2017). Perfectly matched 20-nucleotide guide RNA sequences enable robust genome editing using high-fidelity SpCas9 nucleases. Genome biology 18, 191.
Wang, C., Liu, W., Wang, G., Li, J., Dong, L., Han, L., Wang, Q., Tian, J., Yu, Y., Gao, C., et al. (2017). KTN80 confers precision to microtubule severing by specific targeting of katanin complexes in plant cells. The EMBO journal 36, 3435-3447.
Meng, X., Yu, H., Zhang, Y., Zhuang, F., Song, X., Gao, S., Gao, C., and Li, J. (2017). Construction of a Genome-Wide Mutant Library in Rice Using CRISPR/Cas9. Molecular plant 10, 1238-1241.
Yin, K., Gao, C., and Qiu, J.L. (2017). Progress and prospects in plant genome editing. Nature plants 3, 17107.
Ran, Y., Liang, Z., and Gao, C. (2017). Current and future editing reagent delivery systems for plant genome editing. Science China Life sciences 60, 490-505.
Zhang, Y., Bai, Y., Wu, G., Zou, S., Chen, Y., Gao, C., and Tang, D. (2017). Simultaneous modification of three homoeologs of TaEDR1 by genome editing enhances powdery mildew resistance in wheat. The Plant journal 91, 714-724.
Li, J., Zhang, H., Si, X., Tian, Y., Chen, K., Liu, J., Chen, H., and Gao, C. (2017). Generation of thermosensitive male-sterile maize by targeted knockout of the ZmTMS5 gene. Journal of genetics and genomics 44, 465-468.
Zong, Y., Wang, Y., Li, C., Zhang, R., Chen, K., Ran, Y., Qiu, J.L., Wang, D., and Gao, C. (2017). Precise base editing in rice, wheat and maize with a Cas9-cytidine deaminase fusion. Nature biotechnology 35, 438-440.
⠂Two recommendations in F1000 Prime
⠂Cover story and Issue highlights
⠂Commentary in Nature Plants by Q. Shan and D. F. Voytas
⠂Commentary in Nature Biotechnology by A. May
⠂Commentary in Trends in Plant Science by M. Marzec and G. Hensel
Liang, Z., Chen, K., Li, T., Zhang, Y., Wang, Y., Zhao, Q., Liu, J., Zhang, H., Liu, C., Ran, Y., et al. (2017). Efficient DNA-free genome editing of bread wheat using CRISPR/Cas9 ribonucleoprotein complexes. Nature communications 8, 14261.
⠂One recommendation in F1000 Prime
⠂Research Highlights in Genome Biology by F. Wolter and H. Puchta
Liang, Z., Zong, Y., and Gao, C. (2016). An Efficient Targeted Mutagenesis System Using CRISPR/Cas in Monocotyledons. Current protocols in plant biology 1, 329-344.
Zhang, Y., Li, J., and Gao, C. (2016). Generation of Stable Transgenic Rice (Oryza sativa L.) by Agrobacterium-Mediated Transformation. Current protocols in plant biology 1, 235-246.
Li, J., Meng, X., Zong, Y., Chen, K., Zhang, H., Liu, J., Li, J., and Gao, C. (2016). Gene replacements and insertions in rice by intron targeting using CRISPR-Cas9. Nature plants 2, 16139.
Zhang, Y., Liang, Z., Zong, Y., Wang, Y., Liu, J., Chen, K., Qiu, J.L., and Gao, C. (2016). Efficient and transgene-free genome editing in wheat through transient expression of CRISPR/Cas9 DNA or RNA. Nature communications 7, 12617.
Gao, C. (2015). Genome editing in crops: from bench to field. National science review 2, 13-15.
Chen, K., and Gao, C. (2015). Targeted Gene Mutation in Plants. Somatic Genome Manipulation: Advances, Methods, and Applications pp. 253-272.
Chen, K., and Gao, C. (2015). Developing CRISPR Technology in Major Crop Plants. Advances in New Technology for Targeted Modification of Plant Genomes pp. 145-159.
Shan, Q.W., and Gao, C.X. (2015). Research progress of genome editing and derivative technologies in plants. Hereditas 37, 953-973.
Ji, X., Zhang, H., Zhang, Y., Wang, Y., and Gao, C. (2015). Establishing a CRISPR-Cas-like immune system conferring DNA virus resistance in plants. Nature plants 1, 15144.
Wang, S., Li, S., Liu, Q., Wu, K., Zhang, J., Wang, S., Wang, Y., Chen, X., Zhang, Y., Gao, C., et al. (2015). The OsSPL16-GW7 regulatory module determines grain shape and simultaneously improves rice yield and grain quality. Nature genetics 47, 949-954.
Shan, Q., Wang, Y., Li, J., and Gao, C. (2014). Genome editing in rice and wheat using the CRISPR/Cas system. Nature protocols 9, 2395-2410.
Wang, Y., Cheng, X., Shan, Q., Zhang, Y., Liu, J., Gao, C., and Qiu, J.L. (2014). Simultaneous editing of three homoeoalleles in hexaploid bread wheat confers heritable resistance to powdery mildew. Nature biotechnology 32, 947-951.
⠂Two recommendations in F1000 Prime
⠂News & Views byJ. Gil-Humanes and D. F. Voytas inNature Biotechnology
⠂Research Highlights in Nature 2014
⠂Commentary by L. DeFancesco, C. Schmidt and E. Waltz in Nature Biotechnology
Xiao, J., Xu, S., Li, C., Xu, Y., Xing, L., Niu, Y., Huan, Q., Tang, Y., Zhao, C., Wagner, D., et al. (2014). O-GlcNAc-mediated interaction between VER2 and TaGRP2 elicits TaVRN1 mRNA accumulation during vernalization in winter wheat. Nature communications 5, 4572.
Voytas, D.F., and Gao, C. (2014). Precision genome engineering and agriculture: opportunities and regulatory challenges. PLoS biology 12, e1001877.
Liang, Z., Zhang, K., Chen, K., and Gao, C. (2014). Targeted mutagenesis in Zea mays using TALENs and the CRISPR/Cas system. Journal of genetics and genomics 41, 63-68.
Gao, C., and Nielsen, K.K. (2013). Comparison between Agrobacterium-mediated and direct gene transfer using the gene gun. Methods in molecular biology 940, 3-16.
Li, J., Zhang, Y., Chen, K.L., Shan, Q.W., Wang, Y.P., Liang, Z., and Gao, C.X. (2013). CRISPR/Cas: a novel way of RNA-guided genome editing. Hereditas 35, 1265-1273.
Shan, Q., Wang, Y., Li, J., Zhang, Y., Chen, K., Liang, Z., Zhang, K., Liu, J., Xi, J.J., Qiu, J.L., et al. (2013). Targeted genome modification of crop plants using a CRISPR-Cas system. Nature biotechnology 31, 686-688.
⠂Commentary in Science by E. Pennisi
⠂Commentary in Science by J. Cohen